protein purification, peptide purification, desalting, pre-concentration, LC MS, sample preparation

Fast, fail-safe purification of peptide and protein samples

"We changed to StageTips for desalting of peptides prior to mass spectrometry analysis because they showed superior binding and recovery of peptides compared to our previous solution from a market leading supplier."  

Dr. Ulrike Resch, Institute for Genetics, University of Cologne, Germany

Highest quality results are critically dependent on optimal sample preparation. The StageTip range offers ready to use, prepacked tips for desalting, pre-concentration, and separation by step elution.

Select C8, C18 or SCX (cation exchange) chromatography media

  • Achieve excellent sensitivity in the low femtomole to high attomole range
  • Follow a simple, effective generic protocol
    • developed to ensure efficient binding  in a single pass

    • load, wash and elute at >300µl/min using low back pressure
  • Ensure high reproducibility
    • no tip variation, reliable protocols and superb ease of use
    • carefully selected media ensure minimal unspecific and irreversible binding

Examples of recent publications

Journal of Experimental Biology 212, 184-193, December 2009
Regional variation in parvalbumin isoform expression correlates with muscle performance in common carp (Cyprinus carpio)

Philip Brownridge1, Luciane Vieira de Mello2, Mary Peters1, Lynn McLean1, Amy Claydon1, Andrew R. Cossins1, Phillip D. Whitfield1 and Iain S. Young1

1Faculty of Veterinary Science, University of Liverpool, Liverpool, UK, 2School of Biological Sciences, University of Liverpool, Liverpool, UK

J. Proteomics 72(6):1061-70, August 2009
pI-based fractionation of serum proteomes versus anion exchange after enhancement of low-abundance proteins by means of peptide libraries

Umberto Restuccia1, Egisto Boschetti 2, Elisa Fasoli, Frederic Fortis2, Luc Guerrier2, Angela Bachi1, Alexander V. Kravchuk3 and Pier Giorgio Righetti3

San Raffaele Scientific Institute, 20132 Milano, Italy, 2 Bio-Rad Laboratories, 92430 Marnes-la-Coquette, France, 3 Department of Chemistry, Materials and Chemical Engineering "Giulio Natta", Politecnico di Milano, 20131 Milano, Italy

Mol Cell Proteomics. 2009 Aug 2. [Epub ahead of print]
Proteomic analysis of microtubule associated proteins during macrophage activation

Patel PC1, Fisher KH 2,3, Yang EC4, Deane CM2,3, Harrison RE1.

1 Department of Biological Sciences, University of Toronto Scarborough, 1265 Military Trail, Toronto, Ontario, M1C 1A4, Canada, 2Department of Statistics, University of Oxford, Oxford, United Kingdom, 3Oxford Doctoral Training Centre, University of Oxford, Oxford, United Kingdom, 4Proteomics Core Facility, Sunnybrook Research Institute, Sunnybrook Health Sciences Centre and University of Toronto, Toronto, ON, Canada.

Mol Cell Proteomics. 2009 Aug 1. [Epub ahead of print]
Differential 14-3-3-affinity capture reveals new downstream targets of PI 3-kinase signalling

Fanny Dubois, Franck Vandermoere, Aurélie Gernez, Jane Murphy, Rachel Toth, Shuai Chen, Kathryn M. Geraghty, Nick A. Morrice, and Carol MacKintosh

MRC Protein Phosphorylation Unit, University of Dundee, Dundee, Scotland DD6 9HX

Journal of Bacteriology, p. 494-505, Vol. 191, No. 2, January 2009
The Peptidoglycan Sacculus of Myxococcus xanthus Has Unusual Structural Features and Is Degraded during Glycerol-Induced Myxospore Development

Nhat Khai Bui1, Joe Gray2, Heinz Schwarz3, Peter Schumann4, Didier Blanot5, and Waldemar Vollmer1

Institute for Cell and Molecular Biosciences, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, United Kingdom,1 ICaMB Pinnacle Lab, Newcastle University, Framlington Place, Newcastle upon Tyne NE2 4HH, United Kingdom,2 Max Planck Institute for Developmental Biology, Spemannstrasse 35, 72076 Tübingen, Germany,3 DSMZ-Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Inhoffenstraße 7B, 38124 Braunschweig, Germany,4 Enveloppes Bactériennes et Antibiotiques, IBBMC, UMR 8619 CNRS, Université Paris-Sud, Orsay, France5

J. Biol. Chem. 283(27):18530-7, July 2008
The transfer of heavy chains from bikunin proteins to hyaluronan requires both TSG-6 and HC2.

Kristian W. Sanggaard1, Carsten S. Sonne-Schmidt1, Toke P. Krogager1, Karen A. Lorentzen1, Hans-Georg Wisniewski2, Ida B. Thøgersen1 and Jan J. Enghild1,2

1Center for Insoluble Protein Structures (inSPIN) and Interdisciplinary Nanoscience Center (iNANO) at the Department of Molecular Biology, Science Park, University of Aarhus, Gustav Wiedsvej 10C, 8000 Aarhus C, Denmark and 2Department of Microbiology, New York University School of Medicine, New York, New York 10016

J. Biol. Chem. 280(45):37803-13. November 2005
Characterization of the role of the Rab GTPase-activating protein AS160 in insulin-regulated GLUT4 trafficking

Mark Larance1,2,Georg Ramm1, Jacqueline Stöckli1, Ellen M. van Dam1, Stephanie Winata1, Valerie Wasinger2, Fiona Simpson3, Michael Graham4, Jagath R. Junutula5, Michael Guilhaus2 and David E. James, A National Health and Medical Research Council senior principal research fellow1

1Diabetes and Obesity Program, Garvan Institute of Medical Research, Sydney 2010, Australia, 2Bioanalytical Mass Spectrometry Facility, University of New South Wales, Sydney 2052, Australia, 3Institute for Molecular Biology, University of Queensland, Brisbane 4072, Australia, 4Benitec, Inc., Mountain View, California 94043, and 5Genentech Inc., South San Francisco, California 94080